The Rotor-Gene 6000 supports four different reaction tube formats to suit different needs and personal preferences. Each format is described below. Changing formats is a simple click-in, click-out change of the metal rotor that holds the tubes. Before starting a run, the software prompts you to identify the tube format and the reaction volume. The rest is automatic, making format change on the Rotor-Gene the simplest of any real-time system.
All formats can be automated using the CAS-1200 personal robotic PCR setup workstation.
Format: Reaction Volume:
Cap Type: Rotor Capacity: Rotor: Locking Ring: Additional Info:
RotorDiscTM 100, P/N 6001-012 5µL-25µL (range) 10µL-25µL (recommended) Heat-sealed plastic film, P/N 6001-013 100 wells Gold, P/N 6001/007 Gold, P/N 6001-008 Useful for high throughput or low-volume reactions. Supports 96-well workflow with extra room for controls.
Format: Reaction Volume:
Cap Type: Rotor Capacity: Rotor: Locking Ring: Additional Info:
RotorDiscTM 72, P/N 6001-015 10µL-50µL (range) 15µL-50µL (recommended) Heat-sealed plastic film, P/N 6001-013 72 wells Platinum, P/N 6001-014 Platinum, P/N 6001-006 Rotary plate equivalent of the 0.1 mL strip tubes (below right). Format used for OTV thermal testing and calibration.
Format: Reaction Volume:
Cap Type: Rotor Capacity: Rotor: Locking Ring: Additional Info:
0.1 mL strip tubes and caps, P/N 3001-002 10µL-50µL (range) 10µL-30µL (recommended) Separate strip caps 72 wells Blue, P/N 6001-001 Silver, P/N 6001-002 Strips of 4 tubes makes handling easy, Matching strip caps have long frosted tabs for convenient labelling and handling.
Format: Reaction Volume:
Cap Type: Rotor Capacity: Rotor: Locking Ring: Additional Info:
Standard 0.2 mL PCR style tubes, P/N 3001-001 10µL-100µL (range) 15µL-30µL (recommended) Attached caps, flat or domed, clear or frosted 36 wells Red, P/N 6001-003 Silver, P/N 6001-004 Useful for larger reaction volumes. Special optical caps are not required as detection is through the base of the tube.
